Can I use this plugin for commission payments in YITH Multi Vendor?

Yes, it’s possible. You can use these two plugins together.

The integration with Multi Vendor allows the admin to split the price of one or more products between the vendor and the admin immediately after the checkout and based on the commission rate that you’ve agreed with them.

I do not have a Stripe account, do I need one?

Yes, both admin and receiver will need to have a Stripe Account in order to make the plugin work.